Design and Application of Four Oligonucleotide Probes Specific for Thermus species
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چکیده
منابع مشابه
Genus-specific polymerase chain reaction for the mycobacterial dnaJ gene and species-specific oligonucleotide probes.
Identification of tuberculous and nontuberculous mycobacteria by biochemical methods is a long-term process that takes up to 8 weeks for completion and requires expertise to interpret the results. In order to detect and differentiate the major pathogenic mycobacterial species, we developed genus-specific primers that amplify the dnaJ gene from the broad spectrum of mycobacterial species and det...
متن کاملSpecies-specific oligonucleotide probes for five Bifidobacterium species detected in human intestinal microflora.
Portions of the 16S rRNA from closely related species of the genus Bifidobacterium that are found in the human intestinal microflora were sequenced in order to design species-specific oligonucleotide probes. Five oligonucleotide probes ranging from 16 to 19 bases in length and complementary to 16S rRNA sequences from Bifidobacterium adolescentis, B. bifidum, B. breve, B. infantis, and B. longum...
متن کاملSequence specific sensitivity of oligonucleotide probes
Gene expression analysis by means of high-density-oligonucleotide-array chips is based on the sequence specific binding of mRNA to oligonucleotide probes and its measurement using fluorescence labels. The sensitivity of a probe to detect a certain amount of target mRNA considerably varies among the about 400.000 probes, which are covalently attached to a typical Affymetrix chip. We present a mo...
متن کاملDesign and application of new 16S rRNA-targeted oligonucleotide probes for the Azospirillum-Skermanella-Rhodocista-cluster.
The genera Azospirillum, Skermanella and Rhodocista form a phylogenetic subgroup within the alfa subclass of Proteobacteria. Based on comparative 16S rRNA sequence analysis a nested set of new oligonucleotide probes was designed. It comprises probes for the whole genus cluster Azospirillum-Skermanella-Rhodocista, for the Azospirilli subcluster I including A. lipoferum, A. doebereinerae, A. larg...
متن کاملUsing ITS2 secondary structure to create species-specific oligonucleotide probes for fungi.
Oligonucleotide microarray based on ITS2 rDNA sequences would be extremely useful in identifying fungi within soil samples. However ITS2 contains phylogenetic information and duplication of sequences among taxa make false positive detections likely unless a way could be found to identify taxon-specific portions of the ITS2 sequence a priori. Examination of component ITS2 sequences suggested one...
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ژورنال
عنوان ژورنال: Systematic and Applied Microbiology
سال: 1997
ISSN: 0723-2020
DOI: 10.1016/s0723-2020(97)80072-0